1. Product Overview
DNBelab-D4 digital sample preparation system adopts fully enclosed digital microfluidic technology, integrates multiple functional modules in one instrument, and can complete all library preparation steps in a fully automatic closed manner on a sample preparation card, realize the whole process of closed preparation from nucleic acid to library preparation products (double-stranded DNA library or DNB), and give the concentration of library products at the same time, which can realize whole genome sequencing, metagenome sequencing, targeted sequencing, library conversion and other library construction processes.
2. Product introduction
- Miniaturization The main machine occupies an area of about 0.1 square meters and weighs < 15 kg, which can be flexibly moved and easy to deploy.
- Fully automatic Digital microfluidic technology is used to accurately control microliter droplets, integrate all functional modules required for library construction, realize fully automatic preparation from nucleic acid to DNB and integrate quantitative functions on a sample preparation card.
- Fully enclosed Using the unique technology of sealing liquid to encapsulate the droplets, the droplets are always completely isolated from the outside air during the whole process of library construction, which is equivalent to "no lid" in the whole process, so as to completely avoid the generation of aerosols and achieve "zero" pollution construction.
- Quick library construction saves the switching time and manual transfer time of different equipment by integrating multiple functional modules, realizes the seamless connection between each library construction step, and makes the automatic database construction one step faster. Taking DNBelab-D4RS Fast PCR-FREE Library Preparation Kit V2.0 as an example, the total time from DNA to DNB and DNB quantification on D4 is only 2.5 h.
3.
Performance parameters
| Technical principle |
Fully enclosed digital microfluidic technology |
| Sample throughput |
1-4 samples/round |
| Manual preparation time |
< 15 minutes |
| Adapt the sequencer |
DNBSEQ-E25，DNBSEQ-G99
MGISEQ-200,MGISEQ-2000 |
| Recommended Apps |
Whole genome sequencing, metagenomic sequencing, targeted sequencing, library conversion and other library construction processes |
| Open adaptation |
Support for custom development of third-party kits* |
*The third-party customized development is supported by MGI, which has customized the development of pathogen targeted sequencing, pathogen metagenomic sequencing, tumor panel targeted sequencing, judicial individual identification sequencing and other database construction processes.
4.
System parameters
| Power & Size & Net Weight |
Operating environment & touch screen |
| Host input: 24 VDC, 6 A |
Ambient temperature: 15 °C~30 °C |
| Adapter input: 100 V~240V，50/60 Hz，250 VA |
Relative humidity：20% RH~60% RH，non-condensing |
| Dimensions: 356 mm (length), × 315 mm (width), × 257.5 mm (height) |
Air pressure range: 70 kPa ~106 kPa |
| Net weight: 15 kg |
Touch screen: LCD display, 8 inches, 1024×600 pixels |
